RNA
Part:BBa_K4662013
Designed by: Julia Wyss Group: iGEM23_UZurich (2023-10-02)
crRNA to block Pyruvate formate-lyase 1-activating enzyme
crRNA designed to block the promoter of Pyruvate formate-lyase 1-activating enzyme when tracrRNA is added and expressed with dCas9.
Characterization
Gene Location NC_000913.3:950447
PAM sequence on antisense strand
predicted efficiency: 68%
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Part can be used to manipulate central metabolism leading to more available pyruvate in the cell.
For more information on it's usage BBa_K4662071
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Categories
Parameters
None |